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KMID : 0378019730160070059
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New Medical Journal
1973 Volume.16 No. 7 p.59 ~ p.66
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A Study on the Phagocytic Function of Neurophils in Patients with Leprosy
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金忠相/Kim, Choong Sang
金源錫/林壽德/Kim, Won Suk/--
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Abstract
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A significant increase of dark blue formazan in neutrophils from reduction of nitroblue tetrazolium dye in patients with bacterial infection than those with nonbacterial illnesses or healthy person was first tested in vitro by Park and his associates. Thereafter, the nitroblue tetrazolium dye reduction test (NBT test) has been found an useful diagnostic aid in differentiating certain types of bacterial infection from non-bacterial disease.
The present study was undertaken to determine that the neutrophils in patients with leprosy are responsible for the major role in defence mechanism against Mycobacterium leprae.
The NBT and stimulated NBT test were performed in 25 cases of lepromatous leprosy, 11 cases of tuberculoid leprosy and any 20 cases of healthy controls by Park´s method. Most of the patients with leprosy were receiving antileprosy treatment with diaminodiphenyl-sulfone (DDS) for varying duration except 4 of lepromatous and 1 of tuberculoid leprosy patients.
The results were as follows.
1. The mean percentage and range of NBT-positive neutrophils in patients with lepromatous leprosy, tuberculoid leprosy and healthy control group were 1G.3± 5.0% (1-21%), 9.5%±4.5%(3-19%) and 8.3±2.7%(3-13%), respectively.
The mean absolute number of N13T-positive neutrophils in each group were 482. 1
±224.3, 503.5.:.379.2 and 421.1±227.2, respectively.
2. By the stimulated NBT test, the mean percentage and range of NBT-positive neutrophils were 32.4±10.6% (17-59%), 37.2±9.2% (27-62%), and 30.8±9.7% (21-58%) in patients with lepromatous and tuberculoid leprosy and healthy control groups, respectively. The mean absolute number of NBT-positive netrophils in each group were 1,507.9±590.1, 1,971±263.6 and 1358.21973.4, respectively.
The results showed negative NBT response in both types of leprosy and there was no significant difference in the-values of NBT-positive neutrophils between leprosy and healthy control subjects in our study. These results mean that the neutrophils in patients with leprosy are not responsible for the major role in phagocytosis against Mycobacterium leprae. However, NBT-positive neutrophils in untreated cases were likely to be higher than those in treated cases and healthy control subjects. Further study may be necessary to evaluate the NBT test in untreated patients with leprosy.
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